Advanced Tools for Understanding Biological and Environmental Research
Bei Nie, Ph.D., Professor of Chemistry
Chongqing Institute of Green and Intelligent Technology, Chongqing, P. R. CHINA.
Merck Research Laboratory, Rahway NJ, USA.
3: pm, 21st, December
Meeting Room, First Floor
College of Chemistry and Materials Science
Research in our lab is directed at the development of advanced tools and functional materials to drive biological and environmental research, which can be briefly outlined as below:
Tools for understanding gene variation, gene regulation and proteoform. The successful completion of sequencing the human genome and other genomes ushered in a new era in biological research. A strong focusnowisidentifying individualgeneticvariations,regulatorymechanisms,whicharesignificantly altered by critical biological processes, diseases and environmental factors. Our platform is established on nanocrystalline diamond thin film to fabricate the high-density addressable DNA oligonucleotide array using the maskless light-directed photochemistry, producing the most stable and biocompatible system forgeneticanalysis.Combinedwithsurfaceinvasivecleavageassay,thistechnologyenablesusto decipher hundreds of thousands of genome-wide SNP alleles in a single-pot reaction with the reasonable cost. Beyond this, in the currently ongoing project, we are developing a new strategy for proteoform analysis,inwhichthedeterminationofaccuratemassfrom“top-down”approachwillelucidate comprehensiveproteinvariations,thatinformationusuallyomittedbyprevalent“bottom-up”LC/MS strategy. The combined efforts will thereby make possible critical new insights into the functioning of biological systems and associated pathways.
acicular-Mullite nanocrystal ceramic for catalytic diesel particulate filter. Diesel engine emission counts one-third production of PM2.5, which severely escalates human health and climate risks. Driven by this primary impetus, our study emphasized on developing nanocrystal ceramic substrate for new generation particulate filter, on which electroless-coated platinum catalyst effectively converts particulate matter to gaseousexhaust,regardlessofsurfaceconductivityandchemicalproperty.Interpenetratedcrystal architecture triumphs over the traditional 2-dimensional porous materials by vast surface area, which mounts a greater number of soot and catalysts, and sustains a low backpressure. Both factors are crucial for high efficient combustion engine, friendly to environment.
Nanoporous array in high throughput drug screening.Rapid reaction screening is the pivotal step in small-moleculebaseddrugdevelopment.ConventionalMALDI-basedanalyticalplatformintrinsically suffers deteriorative interference from matrix itself. A nanoporous array made from GaN was developed to meet this peculiar request, on which matrix-free ionization mass spectrometry and surface-enhanced Ramanscatteringwereperformedinorthogonalandcorrelativefashion.Theintegratedreaction-detection system allows rapid monitoring of EOR (end-of-reaction) with a high throughput manner. The coupled studies shed a new insight into the reaction mechanism and catalytic pathway, elucidating the transition of analyte from metal-solution interface to gaseous ions.